Objectives: to examine the opinions of health professionals of the Orašje County Hospital on hand hygiene, self-efficacy and how they self-assessed with regard to hand hygiene based on guidelines. Study design: a cross-sectional study conducted with the help of a questionnaire made only for the purposes of this study during February 2021. Participants and methods: The study had 101 participants. In this research, data processing procedures were used in accordance with the set problems. The value of P < 0.05 was taken as the level of statistical significance. The statistical package IBM SPSS 25, manufactured in Chicago, USA, in 2017, was used for processing. Results: In the study, out of 101 participants, 81 were female (80.2 %) and 20 (19.8 %) were male, and 32 (31.7 %) participants were aged 31 to 40 years. Most participants answered questions that related to general knowledge of hand hygiene correctly. It was found that participants aged 18 to 30 show significantly poorer implementation of hand hygiene, but will significantly more, in situations where there is less work, pay more attention to hand hygiene. Also, they believe that most of their colleagues often follow the hand hygiene recommendation even in difficult situations. Significantly more participants aged 51 and over believe that they are often criticized by colleagues and/or superiors when they do not wash/decontaminate their hands enough. Guidance-based compliance has been noted. Conclusion: It is noticed how the respondents had knowledge about hand hygiene and how long to implement it. Healthcare professionals have rated themselves well with regard to hand hygiene based on the guidelines and have positive attitudes about the implementation of hand hygiene.