Objective of the research: The aim of this study was to examine the physical activity of Physiotherapy students at the Faculty of Dental Medicine and Health Osijek during the COVID- 19 pandemic compared to before the pandemic. Participants and methods: The study was conducted on a total of 118 undergraduate and graduate students of Physiotherapy (31 men and 87 women). Data were collected through an online questionnaire consisting of four parts. The first part of the questionnaire referred to the socio-demographic data of students, while the second and third parts of the questionnaire referred to physical activity before and during the pandemic. The final part examined the students’ attitude about the impact of the pandemic on the level of their overall health and changes in body weight during the pandemic. Results: Data from 118 students, 68 undergraduate and 50 graduate students of Physiotherapy with an average age of 24.15 were analyzed. Statistical analysis indicated that there is no statistically significant difference between the number of physically active respondents before the pandemic and during the pandemic. There was also no statistically significant difference in the number of hours spent sitting, standing, and walking before the pandemic and during the pandemic. During the pandemic, the number of students who exercised in a group decreased, and the number of those who exercised through apps or online increased, as did the number of those who exercised at home. The students managed to maintain the same level of health and maintain their body weight, which can be attributed to the fact that they mostly remained active during the pandemic. Conclusion: During the COVID-19 pandemic, it was observed that students were more active than before the pandemic. The pandemic caused changes in the way physical activity was performed, but no statistically significant difference was observed in the number of hours spent doing physical activity.