Introduction:Spiritual care in nursing is defined as help that nurses provide related to religious and existential needs of patients, including their questions about experiences of meaning and purpose of existence. Objectives:To examine the perception of nurses towards spirituality at the Osijek Pediatric Clinic. Study Design:Cross-sectional study conducted during March and April 2022. Participants and Methods:103 nurses/technicians of the KBC Osijek Pediatric Clinic filled out a questionnaire that contained the sociodemographic data of the respondents, the SSCRS scale for evaluating spirituality and spiritual care, the JAREL SWBS spiritual well-being scale, and questions from nursing practice about the ability to provide spiritual care and educational opportunities. Results:According to the areas of spirituality and spiritual care, the perception of individualized care is the highest, and the perception of religiosity is the lowest. The largest number of respondents belong to the high and medium category of spiritual well-being. Respondents who have 30 or more years of service and belong to the older age group have a significantly higher perception of religiosity, while respondents who do not practice their religion have a significantly lower perception of religiosity. Atheists have a marginally significantly lower perception of the area of individualized care. Respondents who are considered sufficiently qualified to assess spiritual care have a significantly higher perception of the area of spirituality, spiritual care and individualized care. Conclusion:This research confirmed that nurses' own perception of spirituality helps in better assessment of patients' spiritual needs, and the lack of competence, knowledge, time and staff are the main obstacles in achieving succesful implementation of spiritual care. The current formal education of nurses is not sufficient for the development of spiritual care competencies. The education of nurses about spirituality is a key factor for the successful implementation of spiritual care in patients.