The aim of the study: To examine the frequency of application of intravitreal injections before and during the COVID-19 pandemic at the Ophthalmology Clinic of the Clinical Hospital "Sveti Duh" in a defined period of time. Research design: This research was conducted as a retrospective cohort study. Participants and methods: The study included 1,975 subjects of both sexes, of which 911 were female and 1,064 were male. They were treated with intravitreal injections at the Ophthalmology Clinic of the Clinical Hospital "Sveti Duh". Data was collected retrogradely from the Hospital Information System (BIS) for the period from 18/08/2019 to 14/09/2020, based on the following criteria: use of intravitreal injection more than once in one or both eyes, and use of Eylea, Lucentis, Avastin or Kenalog. Results: In the sample, significantly more intravitreal injections were administered in the observed period after the declaration of the pandemic in the Republic of Croatia, 1311 of them (65.6 %) (Chi square test; P < 0.001). The results showed that before the COVID 19 pandemic, age was significantly less associated with the best corrected visual acuity (Spearman's correlation; P < 0.001) and the central thickness of the macula (Spearman's correlation; P = 0.04), i.e. the older the subject's age, the smaller the best corrected visual acuity and the central thickness of the macula. Also, before the COVID 19 pandemic, the age has a significantly low correlation with the best corrected visual acuity (Spearman's correlation; P < 0.001), i.e. the older the subject's age, the lower the best corrected visual acuity. Conclusion: Based on the conducted research and obtained results, we can conclude that the frequency of application of intravitreal injections is higher after the declaration of the COVID pandemic compared to the period before the pandemic, and that there is a significant difference in the type of injection administered before and during COVID.