Aim of the research: To examine the level of knowledge and opinion of nurses about professional communication, to examine whether there are differences in knowledge and opinion of nurses about professional communication in relation to age, level of education and work experience Research design: Cross-sectional study Respondents and methods: The research included 60 respondents, nurses/technicians employed in the Osijek-Baranja County Health Center, in the patronage service. There were 32 (53 %) bachelor's degree holders, 27 (45 %) master's degrees in nursing and 1 (2 %) graduate nurses/technicians. For the purposes of this research, a questionnaire was created by the author, which consists of three parts. The first part consists of demographic data. The second part consists of thirty statements to which respondents should have circled T (true) or N (false), if they disagree with the statement. The third part of the questionnaire consists of five statements to which respondents had to answer, to what extent they agree or disagree with a particular statement. Results: In the research, the majority of respondents were female, 59 (98%), aged 31 to 45, according to their place of work, 25 (42%) of them work in the city. The median number of correct answers on the assessment of communication knowledge is 24 (interquartile range 23- 25) ranging from a minimum of 18 to a maximum of 27 correct answers. There are no significant differences in the respondents' knowledge of communication with regard to the characteristics of the respondents. Master's degrees, compared to bachelor's degrees, estimated more that they are not sufficiently educated to communicate with specific groups of patients p = 0.04. Conclusion: In order to improve professional communication in the work of nurses, professional education on communication is needed, it is necessary to conduct regular courses for all health personnel involved in patient care.