Objective: To examine the opinion of nursing students of the Faculty of Dental Medicine and Health on lifelong learning; whether there are differences in: motivation, inclination towards research and acquisition of knowledge and skills, skepticism and curiosity versus demographic variables. Study design: A cross-sectional study was conducted. Respondents and methods: The participants of the research are students of undergraduate and graduate university studies in Nursing at FDMH. A total of 128 students participated; 111 women and 17 men. A questionnaire of 43 questions was used. Descriptive statistics methods were used to describe the frequency distribution. The Kruskal Wallis test was used for the difference between several independent variables, while the Mann Whitney test was used for two independent variables. Results: All respondents believe that continuous education and permanent training are necessary in nursing. There is no significant difference in students' motivation and inclination towards research and acquisition of knowledge according to demographic variables. Respondents who live in urban areas express significantly greater skepticism towards the spread of knowledge and skills (P = 0.01). Students aged 18 to 25 (P = 0.007), singles (P = 0.01) and those in the first year of undergraduate studies (P = 0.04) have a significantly greater lack of curiosity for lifelong learning. Respondents who attend some education have a significantly greater inclination towards research and acquisition of knowledge and skills (P < 0.001). Conclusion: There is a need for lifelong learning in nursing. Students' opinions about lifelong learning are of great importance because they can provide insights into the direction in which learning, education, and lifelong learning programs can be improved and adapted.