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undergraduate thesis
In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans
2021. urn:nbn:hr:243:066061
Aleksić, Anamarija
Josip Juraj Strossmayer University of Osijek
Faculty of Dental Medicine and Health Osijek

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Aleksić, A. (2021). In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:243:066061

Aleksić, Anamarija. "In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:243:066061

Aleksić, Anamarija. "In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:243:066061

Aleksić, A. (2021). 'In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:243:066061

Aleksić A. In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek; 2021 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:243:066061

A. Aleksić, "In vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Dental Medicine and Health Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:243:066061

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Metadata
TitleIn vitro ispitivanje utjecaja oralnih sredstava za ispiranje usne šupljine na sojevima Candida albicans
Title (english)In vitro study of the effect of oral mouthwashes on Candida albicans strains
AuthorAnamarija Aleksić
MentorJasminka Talapko (mentor)
Committee memberŠtefica Mikšić (predsjednik povjerenstva)
Committee memberDavor Seifert (član povjerenstva)
Committee memberMarko Matijević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Dental Medicine and Health Osijek
Osijek
Defense date and country2021-06-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Cilj istraživanja: U ovome znanstveno-istraživačkom radu dokazali smo različito djelovanje vodica za ispiranje usne šupljine na Candidu albicans. Materijali i metode: Ispitivanje je provedeno na sojevima kvasaca Candida albicans koji su dio zbirke sojeva Laboratorija za mikrobiologiju, Fakulteta za dentalnu medicinu i zdravstvo Josipa J.J. Strossmayera u Osijeku. Vodice za ispiranje usne šupljine koje smo koristili su: Belosept, Listerine, Curasept, Dontodent, Perio Aid i Oral B. Antifungalno djelovanje vodica ispitivali smo metodom disk difuzije s jažicama. Nakon priređenih suspenzija kvasaca, rotiranjem ploče u tri smjera uz pomoć sterilnih briseva nanijeli smo ih na Müller-Hintonov agar. U šest od sedam jažica dodane su različite vodice, a u sedmu je dodana fiziološka otopina (negativna kontrola). Zatim su stavljeni sojevi u aerobnim uvjetima na inkubaciju 48h na temperaturi od 36° C. Analiza varijance i Studentov t-test korišteni su za utvrđivanje antifungalnog djelovanja vodica te za njihovu međusobnu razliku u antifungalnom djelovanju. Rezultati: Dobiveni rezultati pokazali su da najveće antifungalno djelovanje na sojeve Candida albicans ima Perio Aid, dok Listerine nije imao nikakvo. Također, najveću zonu inhibicije imao je Perio Aid (od 24 mm do 37 mm), dok je najmanju imao Oral B (od 23 mm do 30 mm). Zaključak: Sve su vodice za ispiranje usne šupljine pokazale antifungalno djelovanje u odnosu na negativnu kontrolu osim Listerina, a u odnosu na pozitivnu kontrolu (amfotericin B) samo Belosept nije pokazao bolje antifungalno djelovanje. Bolje rezultate pokazale su vodice na bazi aktivnog sastojka klorheksidina (ili i cetilpiridinijeva klorida) u usporedbi s vodicama samo na bazi cetilpiridinijeva klorida.
Abstract (english)
Objective of the research: In this scientific research work, we proved the different effects of mouthwashes on Candida albicans. Materials and methods: The study was conducted on yeast strains of Candida albicans that are part of the collection of strains of the Laboratory of Microbiology, Faculty of Dental Medicine and Health J. J. Strossmayer University of Osijek. The mouthwashes we used were: Belosept, Listerine, Curasept, Dontodent, Perio Aid and Oral B. The antifungal effect of the mouthwash was examined by the method of disc diffusion with wells. After the yeast suspensions were prepared, they were applied to Müller-Hinton agar by rotating the plate in three directions using sterile swabs. Different mouthwash was added to six of the seven wells, and saline (negative control) was added to the seventh. The strains were then placed under aerobic conditions for 48 h incubation at 36 ° C. Analysis of variance and Student's t-test were used to determine the antifungal activity of the mouthwashes and for their mutual difference in antifungal activity. Results: The obtained results showed that Perio Aid had the greatest antifungal effect on Candida albicans strains, while Listerine had none. Also, Perio Aid had the largest zone of inhibition from 24 mm to 37 mm, while Oral B had the smallest from 23 mm to 30 mm. Conclusion: All mouthwashes showed significant antifungal activity compared to the negative control, and compared to the positive control (amphotericin B) only Belosept did not show better antifungal activity. Mouthwashes based on the active ingredient chlorhexidine (or cetylpyridinium chloride) also showed better results compared to mouthwashes containing only cetylpyridinium chloride.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:243:066061
Study programmeTitle: University Undergraduate Study of Dental Hygiene
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica dentalne higijene (sveučilišni prvostupnik/prvostupnica dentalne higijene)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-07-11 12:10:13