The aim of the research: To examine the opinions of nursing students about transgender people and the differences in opinions according to the demographic characteristics of the respondents. Study design: Cross-sectional study. Respondents and methods: There were 50 respondents who voluntarily agreed to participate in the research. The survey is anonymous and was conducted in June 2023. It took 5 minutes to fill out the questionnaire. Results: Significantly more respondents of unspecified gender, unemployed respondents and respondents aged 18 to 25 know at least one transgender person, while significantly more male respondents and respondents aged 26 to 35 do not. Respondents aged 26 to 35, respondents living in a city with more than 100,000 inhabitants and employed respondents have a more positive opinion regarding the fact that transgender people are people whose identity does not match the gender they were assigned at birth, while respondents aged 18 to 25 and respondents living in cities with 15,000 to 100,000 inhabitants show a more negative opinion. Employed respondents between the ages of 26 and 35 show a more positive opinion about the provision of care, while employed respondents older than 36, who have a secondary education in the field of healthcare show a more positive opinion regarding the approach to transgender people. Unemployed respondents would change their opinion in a negative way regarding a person if they knew they were transgender. Conclusion: There is a need to increase awareness of gender dystrophy, especially in groups of respondents who do not have a secondary education in the field of healthcare and are not employed in the healthcare profession.