The goal of study: The goal of this study is to examine the connection between stress and the quality of life of the health care workers at the Department of emergency medicine in Sisak Moslavina county. The organization of study: A cross-sectional study was conducted. Respondents and methods: 102 health care workers of the Department of emergency medicine of Sisak- Moslavina county took part in the study. Two standardized questionnaires named DASS- 21 and WHOQOL: Measuring Quality of Life were used as a research instrument to examine the presence of stress, anxiety and depression. The results: Most of the respondents are in normal values of the presence of stress, anxiety and depression, that is, a small part of them feel mildly and moderately stressed, anxious and depressed. No statistically significant differences were found regarding age, sex, respondent’s workplace and working experience. The respondents are satisfied with their quality of life without significant differences regarding sex, age and working experience. Regarding respondents’ workplace the quality of life is statistically significantly increased, that is, the drivers show a high level of mental well- being and positive influence of the environment on them, which contributes to a higher quality of life. A negative correlation was found between stress, anxiety, depression and the respondents’ quality of life. It means that the respondents’ quality of life is better if there is less stress, anxiety and depression. There is a connection between stress and satisfaction with the respondents’ quality of life in such a way that a lower level of stress gives a higher quality of life and vice versa. Conclusion: Most of the health workers of the Department of Emergency Medicine are not under the influence of stress, anxiety and depression. They are satisfied with the overall quality of life. The connection between stress and satisfaction with the respondents’ quality of life was established, i.e. the less stressed they are, the higher their quality of life.