Objectives: Research Objective: The aim of the study is to investigate the attitudes and knowledge of graduate nursing students about the National Breast Cancer Early Detection Program and to examine whether they are familiar with the preventive measures for breast cancer and whether they need to upgrade their knowledge. Study design: A cross-sectional study was conducted for the purpose of this study. Subjects and methods: The participants were graduate nursing students at the Faculty of Dental Medicine and Health Osijek. The study involved 70 participants. The questionnaire contained 48 questions. The statistical significance of differences in the total knowledge scale was analyzed using analysis of variance. Descriptive statistics were used for other analyses. Results: A high level of understanding the severity of the disease and its impact on the population was indicated by the high degree of accuracy in answering the question of whether the mortality rate from breast cancer in Croatia is still very high, with 92.9% of respondents answering yes. Participants had less knowledge about the frequency of women being invited for preventive screening within the National Program, with 62.9% of participants giving a correct answer. Knowledge was even lower regarding the expected percentage of positive findings after screening tests (42.9%), the target age group of women covered by the National Program (30.0%), and the annual incidence of breast cancer, i.e., the number of women who annually develop breast cancer in Croatia to which 15.7% provided a correct answer. Conclusion: Participants confirmed that they have knowledge about preventive measures for breast cancer. Participants showed that they should upgrade their knowledge about the National Breast Cancer Early Detection Program.