Stress experienced by nurses/nurse technicians indicates a state of disturbed psychophysical balance. It manifests as emotional exhaustion, depersonalization and decreased personal achievement. Research aim. Determine the intensity of workplace stress, which nurses/nurse technicians experience in operating rooms, determine whether sociodemographic characteristics have a significant role in their experience of stress, as well as to determine whether unforeseen and unplanned daily situations, administrative tasks and conflicts with colleagues have a significant role in workplace stress. Research draft. An average survey was conducted. The criterion for inclusion in the research was the work in the operating rooms of the Clinical Medical Center Osijek. Participants and methods. The survey was conducted in July 2021. A total of 101 nurses/nurse technicians employed in the operating rooms of the CMC Osijek participated in the research. As a research method, anonymous questionnaires were used, which consisted of questions on work intensity and a scale of perceived stress. Results. Some participants, a total of 46 of them, express high levels of stress. There is a significant difference in the perception of stress between men and women in terms of the major stressors. In the correlation of factors, women showed a higher intensity of stress than men. Taking into account the age of the participants, younger ones showed significantly lower intensity of stress in all factors in contrast to older participants who showed higher levels of stress. Conclusion. Looking at the results, we can see the presence of stress in a small proportion of nurses/nurse technicians (a total of 46 of them). This study showed that there is a clear difference in stress experience between men and women. Although it has been proven that everyday unforeseen or unplanned situations, administrative tasks and conflicts with colleagues significantly affect stress, a large number of nurses/nurse technicians believe in their abilities to deal with personal problems.