Objectives: To establish differences in attitudes and knowledge about organ donation among nurses working in surgical and non-surgical departments. Study Design: A cross-sectional study was conducted in Clinical hospital centre Osijek from September to October 2017. Participants and methods: Participants were 223 nurses working in CHC Osijek. The research instrument was an anonymous questionnaire consisting of sociodemographic information and questions on attitudes and knowledge about organ donation. Results: Results show that the majority of participants would donate their organs after death (71,2%). The most frequent reason for not donating organs is lack of information (40%), importance of knowing that their body will be buried as a whole (21,8%), and participants' age (20%). Participants would donate organs in case of a close family member’s death (52,3%). Only 29 participants have a donor card (13,2%), with a significant difference for nurses from surgical departments (P = 0,04). Participants who would not donate their organs replied significantly more often that they either would not receive or do not know whether they would receive an organ from a donor with different religious beliefs (P < 0,001), or that they do not know anyone waiting for a transplant (P = 0,001). Participants from surgical departments (P = 0,004) and those holding an MA in nursing (P = 0,009) gave significantly more correct answers. Conclusion: Majority of participants would donate organs after death, without significant statistical difference with respect to workplace and level of education. Knowledge about organ donation is average, significantly higher among participants holding an MA in nursing and among participants from surgical departments.