Research objective: to examine the knowledge and attitudes of nursing graduate students regarding HPV vaccination. Study design: a cross-sectional study was conducted. Participants and methods: the research was conducted during April and May 2023. It was carried out using an anonymous online survey created on Google Forms. The study involved 60 students from the first and second year of the off-campus graduate program at the Faculty of Dental Medicine and Health Osijek, Sveta Nedjelja. Most of them were second-year students. Results: most participants who took part in the survey had a positive opinion about HPV vaccination, nearly 80% of them. However, despite their positive attitude, many of them had not been vaccinated (95%). Although the knowledge about HPV was very high, the participants believed that HPV was still a taboo topic, and that further education of the population was necessary. Those with more knowledge about HPV usually had a more positive attitude towards it. The results show that students have a positive attitude towards gynecological examinations as a means of prevention and early detection of HPV. They consider it important to practice safe sex regardless of vaccination. Students have a relatively high level of knowledge about HPV and the HPV vaccine. In the future, most of them would agree to vaccinate their own children, although some are unsure about the safety and sufficient research of the vaccine. Whether HPV vaccination should be mandatory or not remains a debatable topic, but the aim is to increase public awareness about the vaccine’s efficacy and thereby promote vaccination and ultimately create “herd immunity”. Conclusion: through the analysis of this research and the obtained results, it can be concluded that students generally have a positive attitude towards HPV vaccination but a low level of vaccine uptake. Moreover, they have a sufficient level of knowledge about the disease itself; however, they believe that there is still room for education and information regarding HPV. To achieve the best possible effect in HPV vaccination rates, it is important to educate the public about the disease and the vaccine, thus encouraging the population to get vaccinated.