Objective: To examine the knowledge and attitudes about breast self-examination among students of Graduate School of Nursing in Sveta Nedelja. Study design: Cross-sectional survey. Subject and methods: the graduate students of nursing in School of Nursing in Sveta Nedelja participated in the research. The research was conducted in March 2023, and 60 respondents participated. The data was collected using an anonymous questionnaire via Google Forms. The collected data included age, employment information, current student status, and education level. Results: The obtained results show that 40% of the respondents perform breast self-examination which is important in preventing cancer. More than half of the respondents were not familiar with self-examination and 43,3% neither agree nor disagree that nurses in Croatia are sufficiently informed about its importance. Healthcare professionals can improve the patient experience by talking to them throughout the process. The workplace of nurses increases the risk of breast cancer, so they must be aware of it. Improving awareness of breast self-examination through training of health professionals can contribute to early diagnosis of breast cancer and better treatment rates. Conclusion: The greater the knowledge about self-examination, the greater the self-assessment of knowledge. The more respondents agree that nurses have introduced them to breast self-examination, the greater their agreement with the statement that nurses in Croatia are sufficiently informed about the importance of breast self-examination and vice versa. More than half of the respondents believe that their knowledge of self-examination is good, which is supported by the correct answers to questions about breast cancer.