Introduction: Healthcare workers are constantly exposed to stress, and the amount of stress is determined by work ing hours, work dynamics, a s well as the con tinuous care for patients. This often leads to additional emotional pressure on healthcare workers, which can negatively affect their mental health and also favors burnout at work work. Objectives: To determine the incidence of burnout syndrome among employees of the Department of Emergency Medicine of the University Hospital Centre Zagreb. The specific objectives are: to determine whether there is a difference in the incidence of burnout syndrome between healthcare workers of all profiles, those who work in shifts and only morning shifts, and also with regard to gender. Study Design: A cross cross-sectional study. Participants and methods: A total of 85 employees of the Department of Emergency Medicine of the University Hospital Centre Zagreb participated in th this research. The research was approved by the Ethics Committee of the University Hospital Centre Zagreb. Respondents were presented with the Copenhagen Burn Burnout Inventory questionnaire , and the study was conducted in the period from March 25 to May 5, 2023. Kolmogorov Kolmogorov-Smirnov test, t-test for independent samples and the ANOVA test were used for statistical analy analysis , and it was done in the statistical software STATISTICA 13 , manufactured by Tibco, California. Results: The research revealed a high occurrence of burnout syndrome among the respondents . There was no statistically significant difference in the incidencincidence among health care workers of different profiles, nor with regard to working hours or gender, except for the incidence of a personal feeling of burnout, which is greater in female respondents (t = 2.95; P = 0.004). Conclusion: Burnout syndrome is a major problem for the healthcare system, and its increasingly frequent occurrence has been proven by other studies . It is necessary to conduct further research that will deal with the mentioned topic . It is crucial tha that healthcare institutions recognirecognize the problem and work to reduce it s occurence occurence.