Objectives: The goals of this research are: 1. to examine whether there is a difference in the quality of life between healthcare professionals who work shift work and those who only work the morning shift 2. to examine whether there is a difference in the quality of sleep between healthcare professionals who work shift work and those who only work in the morning shift Study design: cross - sectional study Participants and methods: The respondents in this research are healthcare professionals of the Dubrava Clinical Hospital employed in the Unified Emergency Care Unit, Department of Urology, Department of Abdominal surgery, Department of Orthopedics and Traumatology and Polyclinic, a total of 100 respondents, divided into two groups, 50 who work only the morning shift and 50 who work shift work. The research methods are questionnaires on the quality of life (WHOQOL-BREF) and the questionnaire on the quality of sleep (PSQI), completed anonymously and voluntarily. Results: 100 respondents participated in the research, healthcare professionals divided into two groups, 50 who work the morning shift and 50 who work shift work. The results obtained through statistical processing indicate a statistically significant difference in the quality of life between the two groups in the form of a poorer quality of life and more difficult organization of free time for employees who work in shifts, as well as poorer quality of sleep in the same group. There is no statistically significant difference in self-assessment of health and concentration in performing daily activities between the two groups. Conclusion: 1. there is a difference in the quality of life between healthcare professionals who work shift work and those who only work the morning shift 2. there is a difference in the quality of sleep between healthcare workers who work shift work and those who only work the morning shift