OBJECTIVES: To examine the opinions and knowledge of students of the University graduate study of Nursing about palliative care. STUDY DESIGN: A cross-sectional survey was conducted. PARTICIPANTS AND METHODS: The respondents were students of the University Graduate Study of Nursing, Faculty of Dental Medicine and Health, Osijek, located in Sveta Nedelja, who voluntarily agreed to participate in the research and fill out the Questionnaire about the opinion and knowledge of Nursing students about palliative care online. RESULTS: There were more female respondents (76,72%), aged 31 and over (57,76%), in the second year of graduate studies, employed and with more than 10 years of work experience. The majority of respondents meet patients in their work who need palliative care, in most cases none of their close people used palliative care services. The majority of respondents are not satisfied with formal education in palliative care, the majority believe that they do not have enough knowledge to provide quality palliative care, the majority do not consider themselves competent to talk to a dying person and the majority have a need for additional education in the field of palliative care. The majority of respondents expressed insufficient (56,03 %) and good (42,24 %) knowledge about palliative care. In their work, women significantly more often encountered patients who needed palliative care, while men were considered significantly more competent to talk to a dying person, no significant correlations were observed between other opinions and demographic characteristics of the respondents. Good knowledge was significantly more frequent in respondents in the second year of the nursing graduate study compared to good knowledge in respondents in the first year of study, and in respondents who were considered competent to talk to a dying person compared to respondents who were not considered competent to talk to a dying person. CONCLUSION: Insufficient knowledge along with dissatisfaction with formal education and the need for additional education indicate the need for significant changes in the formal education of nurses/technicians.