Objectives: To examine the impact of long-term learning and sitting on the locomotor system in students in healthcare studies. Study design: A cross-sectional study. Participants and methods: 142 students of the Faculty of Dental Medicine and Health Osijek participated in the research. Students of the undergraduate and graduate university studies of physiotherapy, nursing, dental hygiene and the integrated university studies of dental medicine participated in the research. The questionnaire was designed to examine the impact of long-term studying and/or sitting on the locomotor system of students at the Faculty of Dental Medicine and Health Osijek. The Chi-square test was used to test the correlation of the variables, while the Mann-Whitney U test was used to test the differences between the variables. Results: Most of the surveyed students experience health problems of the spine after prolonged sitting (83,8 %). Students believe that they have less time for recreation during their studies, and this reduction in free time is statistically significantly associated with health problems of the spine in students (Chi = 14.13; P = 0.003). More than half of students (54,9 %) believe that they have less time for other activities while studying, including sports and recreation. But most students (91,5 %) believe that physical activity helps eliminate and reduce pain. The most common health problem that students feel after prolonged studying and sitting is rigidity. Conclusion: The obtained data indicate a significant impact of long-term studying and sitting on the locomotor system in students. Students who have spent more time sitting have more health problems with the spine and have more severe back pain. Wrist pain was significantly associated with time spent on social media, as well as with the position in which students search social media. Based on these data, it is clear that a sedentary lifestyle harms the locomotor system, especially the cervical spine and lower back.