Research objective: To examine the opinion of the staff of the General Hospital Virovitica on organ donation. Type of research: A cross-section study. Respondents and methods: The respondents are health workers and non-health workers of Virovitica General Hospital. The total number of respondents who completed the voluntary anonymous survey was 103. The respondents were divided into two groups. Healthcare professionals (59) formed the first group and non-healthcare professionals (44) formed the second. The entire research was conducted with the Ethics Committee of General Hospital Virovitica with anonymity guaranteed when completing the survey. Results: The obtained results showed that there is a difference in the attitude and familiarity of employees with regard to the type of work. Healthcare professionals were more knowledgeable compared to non-healthcare professionals, particularly women aged 26-35. Based on the age groups, there was no difference in the attitudes towards organ donation. The examination of views on organ donation according to gender did not reveal diffeference. After the examination, the difference in the familiarity criterion of the position on organ donation was found. There was no difference in the scientific criterion of the position on organ donation based on education background. With regard to marital status, no difference in attitudes was found. Conclusion: Educating the general of all ages, more detailed communication within the family about organ donation, and making important decisions about health conditions create a solid foundation and ensures people cope better with stress in the event of an accident.