Objectives: To examine the quality of life and parental burden in parents of children with developmental disabilities. Study design: Cross-sectional study. Participants and Methods: The study included 113 parents of children with developmental disabilities. The research was conducted in the city of Osijek and towns of Đakovo and Našice using paper and pen method. The data were collected using a questionnaire on general data on parents and children, WHOQOL-BREF questionnaire and Zarit Burden Interview. Results: The quality of life was evaluated as quite good by 49.6 % of parents. Median value of the total scale score for quality of life was 68.2, and the highest score was in the social relationship domain. Physical health had significantly higher score in married respondents, while divorced respondents had significantly lower results in social relationship and in total scale score for quality of life. Physical health had significantly higher score in respondents who did not have any chronic disease. Parents of children with autism spectrum disorder had significantly lower score in the domains of psychological health and environment in comparison to parents of children with multiple diseases. Median of total burden scale was 28. The burden was low to moderate in 51.3 % of respondents and high burden was not experienced by any of the respondents. Mothers experienced higher burden in the domain of loss of control over one's life than fathers. Employed parents had higher burden in financial domain than unemployed parents. Respondents who did not have parent caregiver status had significantly higher burden in emotional well-being and finances domains in relation to respondents who had parent caregiver status. Conclusion: Parents who experienced higher burden had lower quality of life. It is necessary to provide both social and professional support to parents in order to reduce burden and improve the quality of life.