Objectives: To examine the connection between night work, quality of sleep and the life of healthcare employees. To examine the influence of working hours on the quality of sleep, sleepiness and diseases of the respondents, the connection of night work with the domains of mental health, environment, physical health and social relations, the connection of sleep quality and sleepiness in relation to work-related variables, and the mutual connections of the examined domains, sleep quality and sleepiness. Methods: An anonymous and voluntary survey was conducted (July and August 2022) which included 50 respondents. For the survey the WHOQOL-BREF questionnaire, Pittsburgh sleep quality scale and Epworth sleepiness scale were used. The research was conducted on the principle of a cross-sectional study. Results: Significantly more respondents have a worse quality of sleep compared to respondents who show a good quality of sleep (P=0.002). Respondents who have been working in the current way for 26 to 35 years perceive a significantly lower level of quality of social relations compared to respondents who have been working in the current way from 6 to 15 years (P=0.006) and 5 and less years (P=0.02). Significantly more than the expected number of subjects in the total tested sample showed lower daytime sleepiness (P<0.001). Better quality of sleep has a positive effect on increasing the quality of the examined domains. A higher quality of physical health is correlated with an increase in quality of sleep while a poorer quality of sleep is associated with greater sleepiness. Conclusion: The need to encourage healthcare employees to adopt positive lifestyle habits was noted. Managers of health services should try to organize shift work in accordance with individual preferences and in this way influence the increase in satisfaction and quality of life of healthcare employees.