Research Objective: The aim of this research was to analyze the knowledge and opinions of employees of Pregrada High School and Bedekovčina High School regarding the importance of breast self-examination. Study Design: Cross-sectional study. Participants and Methods: The participants were female employees employed at Pregrada High School and Bedekovčina High School. The total number of participants was 70 (N = 70). The research was conducted on participants aged 21 to >51 years and exclusively of the female gender. The total sample consisted of 70 female participants. Data were collected during March, April, and May 2023. For the purposes of the research, an anonymous online survey consisting of 11 questions was designed and used. Results: The results showed that there is a significant difference in the distribution of responses to the question "How often do you perform breast self-examination?" according to the participants' level of education. Furthermore, there is a significant difference in the distribution of responses to the question "When is the best time to perform breast self-examination if you have your period?" according to familiarity with breast self-examination. A significant difference exists in the question "Is it necessary to observe the breasts in the mirror during breast self-examination?". The results showed that there are no significant differences in the distribution of responses to questions related to the assessment of the knowledge and opinions of employees of Pregrada High School and Bedekovčina High School regarding the importance of breast self-examination based on a family history of breast cancer among the participants. Conclusion: Regardless of the level of education of the employees of the high schools who participated in the research, they should participate in the Program in larger numbers and turn breast self-examination into a regular practice of their health behavior.