Objectives: The aim of the research is to examine the level of knowledge of students of the University graduate study in Nursing about palliative care. Study design: Cross-sectional research was applied, with the use of a validated questionnaire. Participants and methods: 55 students of the University graduate study Nursing at the Faculty of Dental Medicine and Health participated in the research. The research instrument was an anonymous questionnaire consisting of two parts. The first part of the questionnaire contained questions about the characteristics of the respondents, and the second part of the questionnaire consisted of the Croatian version of the standardized PCQN questionnaire. Results: Students of the University graduate study in Nursing have a low level of knowledge about palliative care. No significant differences were found in the level of knowledge with regard to the sociodemographic data of the respondents, previous experiences with a dying person and suffered loss of a loved one. Respondents who met patients in need of palliative care in the workplace or clinical exercises had significantly better knowledge about palliative care (P = 0.05). Respondents who think that additional education about palliative care would help them in working with patients have a significantly higher level of knowledge about palliative care, in contrast to respondents who do not know whether additional education would help them in working with patients (P = 0.03). Conclusion: Timely education about palliative care is becoming a necessity of every curricular program. The respondents of this research do not have enough knowledge about palliative care. The level of knowledge was not affected by sociodemographic data. Previous experience with palliative patients and satisfaction with education significantly influenced the level of knowledge about palliative care.