Objectives of the research: The purpose of this research is to examine the knowledge of high school medical students about HPV and vaccination, to investigate the perception of high school students 'knowledge about HPV and vaccination, and to investigate attitudes about high school students' personal decisions about vaccination. Study design: Cross-sectional research. participants and methods: The research was conducted in Pakrac High School in the period from February 20 to April 31, 2020 on a sample of 111 high school students which included high school students, physiotherapy students and nurses and technicians. Exclusively for the needs of this research, a survey questionnaire with twenty questions was created and used. Results: The results of the research speak in favor of the fact that more than 75 % believe that they have an adequate amount of knowledge about HPV infection, which is confirmed by appropriate answers to questions within the questionnaire and that they have gained most of their knowledge on the Internet and through high school education. Currently, about 33 % of high school students are vaccinated against HPV, which corresponds to the average vaccination rate of the state, and more than 60 % of them are planning to be vaccinated in the future. Conclusion: Low percentage of vaccinated students and potential doubts about vaccine safety speak in favor of the need to focus further on additional information of students, parents and educators by a multidisciplinary team of experts through open calls from local Public Health Institutions to help create more positive attitudes and openness towards HPV vaccination in a timely manner.