Research objective: To examine the level of education of physiotherapy students regarding diabetes and its treatment. Study design: Cross-sectional study Respondents and methods: The study included physiotherapy students (N = 47) from the Faculty of Dental Medicine and Health in Osijek, spanning from the first to third year of the undergraduate study programme and the first and second year of the graduate study programme, aged between 19 and 37 years. A questionnaire consisting of 20 questions was developed in the form of a Google Form and was made available online to the participants. The study was conducted in June 2024, and participation was entirely voluntary and anonymous. Results: The data from all 47 respondents were analyzed. The respondents reported acquiring most of their knowledge about diabetes through their university studies (74.47%), websites (53.19%), and social media (31.91%). The data show that the vast majority of respondents (N=13, 27.66%) have a medium level of knowledge, with 10 correct answers out of 18 questions. A statistically significant difference in the level of education about diabetes and its treatment was found with respect to gender (P = 0.05), with female respondents demonstrating a higher level of education. Conclusion: Based on the conducted research, it was determined that students' overall level of education about diabetes and its treatment is medium. A statistically significant difference in education was found concerning the respondents' gender, while no statistically significant difference was found regarding their year of study, engagement in sports activities, or the type of secondary school previously completed.