Objectives: Examine the correlation between stress and the quality of life of nurses and technicians working at the Emergency Medical Services Department of Krapina-Zagorje County. Study design: A cross-sectional study was conducted. Subjects and methods: 30 nurses and technicians working at the Emergency Medical Services Department of Krapina-Zagorje County were included in the research. To conduct the research and create the paper, a questionnaire with predefined answers was designed. The questionnaire consists of a total of 20 questions and statements. The research was conducted using the Google Forms web platform. Participation is voluntary and anonymous. Results: 43.3% of the research participants belong to the age group of 30 - 39 years. The majority are satisfied with the working conditions at the Emergency Medical Services Department of Krapina-Zagorje County. 26.6% of participants feel that their employer does not recognize their efforts at the workplace. 86.6% of employees face stressful situations at work. For 66.7% of participants, working in shifts is not a source of stress. Workplace stress has a negative impact on the quality of life for 36.7% of participants, while 26.7% of them disagree with that statement. Conclusion: Employees of the Emergency Medical Services Department of Krapina-Zagorje County are mostly satisfied with their working conditions. One-third of them believe that their employer does not recognize their efforts at the workplace and that they are not adequately rewarded for their work. Almost all of them face stressful situations on a daily basis. Nevertheless, they do not feel discomfort before going to work and believe they can turn to their colleagues for help. More than half of the participants do not consider shift work to be stressful. Almost all research participants are satisfied with the quality of their life.